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KMID : 0387420190300030199
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Korean Society of Oriental Neuropsychiatry
2019 Volume.30 No. 3 p.199 ~ p.208
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In Vitro Screening of Traditional Medicinal Herbs Combined with Donepezil for Neuroprotective Effects in SH-SY5Y Cells
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Song Sue-Jin
Liu Quan Feng
Hong Min-Ho
Kim Geun-Woo
Koo Byung-Su
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Abstract
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Objectives: The purpose of this study was to evaluate the neuroprotective effects of donepezil and 33 kinds of herbal extract combinations in SH-SY5Y cells with Aβ 25?35 treatment.
Methods: MTT assay was performed to measure the cell viability of each herbal extract combined with donepezil against Aβ-induced neurotoxicity. The most active extracts were then subjected to assess the effects on CREB phosphorylation and COX-2 expressions through the western blot analysis.
Results: There were eight herbal extracts representing significant increase on the cell viability: 1) Erycibe obtusifolia, 2) Polygonum multiflorum, 3) Polygala tenuifolia, 4) Illicium verum, 5) Santalum album, 6) Loranthus parasticus, 7) Platycladus orientalis, and 8) Zanthoxylum piperitum. Especially, when Santalum album and donepezil were treated together, the phosphorylation of CREB significantly increased and COX-2 protein expression was significantly inhibited.
Conclusions: Among the screened herbal extracts, combination treatment of each of the eight herbs and donepezil showed neuroprotective effects in SH-SY5Y cells. Additionally, the combination of Santalum album and donepezil suggested cognitive improvement by up-regulation of p-CREB and down-regulation of COX-2.
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KEYWORD
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Herbal medicine, Donepezil, Drug combinations, Alzheimer’s disease
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